The proposed class action filed in U.S. federal court in Manhattan says Texas-based Alcon Laboratories Inc, which was acquired by Novartis in 2010, maintains a “boy’s club atmosphere” that is hostile to women and bars them from leadership positions.

Novartis spokeswoman Elizabeth Power said Alcon disagreed with the allegations.

“The company is deeply committed to equal employment opportunity for all employees and to preventing discrimination,” Power said.

A U.S. jury in 2010 ordered Novartis to pay more than $250 million in a separate class action that alleged widespread gender discrimination. At the time, it was the largest award in an employment discrimination case in U.S. history.

The company at the time said it would adopt reforms to prevent discrimination and retaliation against employees who complained.

The plaintiffs in Tuesday’s lawsuit, Elyse Dickerson and Susan Orr, say the company violated Title VII of the Civil Rights Act of 1964, which prohibits gender discrimination by employers, and the U.S. Equal Pay Act.

“For years, the company paid them less than similarly situated men, discriminated against them in assignments and other career-enhancing opportunities, and denied them promotions in favor of … men,” the lawsuit says.

Dickerson said she was fired from her post as a global director for complaining about inequities at the company, and is seeking $10 million and her job back.

Orr, a research scientist, said the lack of promotions forced her to resign and is seeking at least $100 million on behalf of a proposed class of thousands of female Alcon employees.

In its most recent annual report released in January, Novartis said it has “continued to focus on the promotion of women.” Tuesday’s lawsuit, however, claims the company has no plan in place to address gender issues at Alcon.

Dickerson and Orr are represented by the national class action law firm Sanford Heisler Kimpel, which brought the earlier lawsuit against Novartis.